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Mattila, P.S., Schugk, J., Wu, H. & Mäkelä, O., 1995 "Extensive allelic sequence variation in the J region of the human immunoglobin heavy chain gene locus", European Journal of Immunology, 25:2578-2582.
Abstract
During the initial stages of B lymphocyte differentiation heavy chain variable (VH), diversity (DH) and joining (JH) gene segments recombine to form a functional heavy chain variable region (VDJ) gene. Evidence for genetic polymorphism of the human JH gene segments has been obtained from mature rearranged VDJ sequences. We conducted an analysis of the published rearrneged JH gene sequences and found that the JH alleles present in the two published germ-line JH region sequences were rare (approx. 2%) in the rearranged sequences. As an attempt to explain this discrepancy a 2.5kb stretch of DNA containing all the six heavy chain JH region genes and the most 3' DH gene segment, DHQ52, was amplified by the polymerase chain reaction from 39 individuals and analyzed for restriction fragment length polymorphism. Five new JH region haplotypes were found and sequenced. These new haplotypes contained the coding segment allels that were frequent in antibody genes. Surpisingly, a high number of interallelic differencies (sic) in the non-coding sequence was found between the new and the two previously published haplotypes implying that the haplotypes had been separated early in evolution. In this respect the JH locus resembles HLA loci.
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Created 27/9/98
Modified 22/7/99